P-376 Restoration of mRNA expression level of endometrial MEIS1, as a co-factor of HOXA10, after laparoscopic salpingectomy of women with hydrosalpinx

Abstract Study question Do salpingectomy could restore HOXA10 co-factor MEIS1 expression in endometrial tissues of hydrosalpinx patients? Summary answer Laparoscopic HOXA10-cofactor- level, addition to HOXA10, women. What is known already Hydrosalpinx defined as a distally blocked and dilated fallopian tube which has been filled with serous fluid. Evidence suggests that associated adverse effect on receptivity by abnormal key molecules such the pre-implantation endometrium. Previous data showed results statistically significant increase expression. three-amino-acid loop extension (TALE) family homeobox gene proven be novel coactivator during human mouse decidualization. MEIS1-HOXA10 complex binds promoters target genes are beneficial for stromal cell design, size, duration This prospective study was conducted from January 2021 2022 determine whether would reverse levels after salpingectomy. All women signed informed consent did not receive any hormonal medication last three months. In this 10 infertile moderate severe hysterosalpingography or laparoscopy undergoing were recruited. Ten healthy fertile age-matched considered control group. Participants/materials, setting, methods women, aged 20-40, sonovisible (diameter >10mm), AMH>1.2 ng/dL, BMI (18–28 kg/m2) regular menstrual cycle indicated mid-luteal progesterone > ng/mL included. history successful pregnancy Mid–luteal-phase sampling (by pipelle) performed at time surgery second obtained forth-post treatment cycle. RNA extraction cDNA synthesis done. Main role chance Quantitative mRNA determined real-time PCR technique. Gene analyzed based 2 –ΔΔCT estimate relative fold change value. The non-parametric Wilcoxon rank test used analysis. P value less than 0.05 significant.There reduction samples group before surgical removal. resulted (about 6-fold) (P = 0.02). Moreover, there difference 0.05). Limitations, reasons caution Larger sample size confirmation these needed. Epigenetic evaluation especially promoter recommended. Wider implications findings restoration observed can molecular mechanism improvement rate IVF Trial registration number applicable